Micro bca protein assay reagent kit, sufficient reagents for 480 tube assays or 3,200 microplate assays. An adaptation of the thermo scientific bca protein assay kit product no. The result is an extremely sensitive colorimetric protein assay in a test tube or microplate assay format. The bca protein assay is a popular method for colorimetric detection and quantitation of total protein. The principle of lowry assay, biuret assay, and bicinconinich bca assay protein assays duration. Micro bca protein concentration assay use the thermo scientific micro bca protein assay kit. This protocol makes use of thermo scientifics bca protein assay in a 96well tissue culture plate format to measure the amount of protein in small volumes of sample. Micro bca protein assay kit is a readytouse, detergentcompatible, western blot related, total protein analysis reagent, used for the quick determination of total protein concentration of dilute protein solutions by measuring absorbance at 562 nm and comparing to a protein standard absorbance vs. If precise quantitation of an unknown protein is required, it is advisable to select a protein. The serva bca protein micro assay kit contains well standardized reagent s which give a linear increase in the reaction product at 562 nm with increasing protein concentrations over a broad range from 0. The assay is derived from the bradford method 1976 and is based on the color change of coomassie brilliant blue g250 dye in direct response to various. Micro bca protein assay kit, sufficient reagents for 480 tube assays or 3200 microplate assays. This kit was previously called bca protein quantification kit.
The pierce bca assay can be used to assess yields in whole cell lysates and affinitycolumn fractions, as well as to monitor protein contamination in industrial applications. Pierce bca protein assay kit thermo fisher scientific. The micro bca protein assay kit from pierce biotechnology is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. The micro bca protein assay kit provides an ultrasensitive method to detect and quantify protein concentration. Mixing together the three micro bca reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The pierce micro bca protein assay kit is a threecomponent version of our bca reagents, optimized to measure total protein concentration of dilute protein.
When stored properly, the kit is stable for 1 year. Biomedical and biological sciences 61,514 views 24. The bca protein assay is suitable for measuring protein concentration in the range of 0. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional differences, providing greater protein to protein uniformity. Both a standard assay for concentrated proteins and a micro assay for dilute protein solutions are described below. Biorad protein assay kit the simplicity, sensitivity, and color of the biorad protein assay make it a great vehicle for integrating biology, math, experimental design, and technique.
The unique, patented method uses bicinchoninic acid bca as the detection reagent for. Protein assays standards, protein assays, orochem, 10pkg. Micro bca protein assay kit thermo fisher scientific. The 1980s saw the introduction by paul smitha of a colorimetric protein assay derived on the biuret reaction using the bicinchoninic acid, known as the bca.
Use the thermo scientific micro bca protein assay kit. Principle bca serves the purpose of the folin reagent in the lowry assay, namely to react with complexes between copper ions and peptide bonds to produce a purple end product. Up67251a reagent a, 250ml up67252a reagent b, 250ml up67253a reagent c, 12ml. Micro bicinchoninic acid bca protein assay gbiosciences. Pierce microplate bca protein assay kit fisher scientific. The takara bca protein assay kit is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. The micro bca protein assay kit is a specialized version of the popular pierce bca protein assay for determining the protein concentration of dilute samples. Bovine gamma globulin kit i, catalog number 5000001 or bovine serum albumin kit ii, catalog number 5000002. The kit has been optimized for determining total protein content in low protein concentration samples 0. Micro bca protein assay kit is a specialised version of pierce bca protein assay for determining the protein concentration of dilute samples. Up75860a microbc assay protein quantitation kit, sufficient reagents for 500testtube or 3 400 microplate assays contains. Smith at the pierce chemical company, now part of thermo fisher scientific, is a biochemical assay for determining the total concentration of protein in a solution 0. Bicinchoninic acid protein assay kit bca1 technical. Thermo scientific pierce bca protein assay kit bca protein.
Bca protein assay reagent bicinchoninic acid youtube. The pierce micro bca protein assay reagent kit is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. Pierce microplate bca protein assay kit reducing agent compatible, sufficient. The amount of purplecolored product formed during the reaction can be correlated to the amount of protein in the bca protein assay.
Pierce bca protein assay kit, sufficient reagents for 250 testtube or 2500 microplate assays. Compared to most dyebinding methods, the bca assay is affected much less by protein compositional differences, providing greater proteintoprotein uniformity. Colorimetric protein assays, such as the coomassie blue g250 dyebinding bradford and bicinchoninic acid bca assays, are commonly used to quantify protein concentration. The micro bca protein assay primarily relies on two reactions. Do triplicates of all samples including the standards. What is the difference between bca and micro bca assay. Compatibility chart for bca kit the amount listed is the maximum amount of material allowed in the protein sample without causing a noticeable interference, when 20 ul protein sample is used for bca assay as described in the standard assay. Incompatible substances amount compatible buffer systems nacetylglucosamine 10 mm in pbs, ph 7. Micro bca protein assay kit the orochem micro bca protein assay kit is a detergentcompatible bicinchoninic acid formulation optimized for the colorimetric detection and quantitation of total protein concentration of dilute protein solutions 0. Pierce micro bca protein assay kit, working range of 0. Determination of dissociation constant of lysozyme binding to tio2 or wo3 lysozyme was dissolved in water to give concentrations of 0, 5, 10, 20, 30, 40, 50, 60, and 70. The dye reagent concentrate can be purchased in a kit with one of two standards. The color produced is stable and increases in a linear fashion over a broad working range of increasing protein concentration.
The thermo scientific micro bca protein assay kit is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of. Since bca is stable, it is incorporated in the reagent formulation at. Pierce bca protein assay kit university of arizona. Smart bca protein assay kit goffin molecular technologies. Micro bca protein assay kit pierce micro bicinchoninic. Instructions micro bca protein assay kit pdf book manual. The micro bicinchoninic acid bca protein assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and is a modification of the bicinchoninic acid bca protein assay for dilute protein samples 0. Pierce microplate bca protein assay kit reducing agent compatible, sufficient reagents for. Microbc assay, micro bca, protein assay dosage quantitation kit bicinchoninic. Albumin standard 0225 0023209 0023210 0023210b 1856269 1878740 nci2320 11252009. The micro bca protein assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions.
How to calculate concentration using the microbca kit. Micro bca protein assay kit 23235 was purchased from. Pipette 25 l of each standard or unknown sample replicate into a microplate well workin g range. Thermo scientific pierce protein assay technical handbook. Us4839295a measurement of protein using bicinchoninic. An adaptaon of the bca protein assay kit, the micro bca kit has been opmized for use with dilute protein samples 0. An adaptation of the bca protein assay kit product no. Product information sheet micro bca protein assay kit. Bradford method utilizes coomassie brillant blue g250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595 nm.
Pierce micro bca protein assay kit biocompare product. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional differences, providing greater proteintoprotein uniformity. Pierce microplate bca protein assay kit reducing agent. Albumin standard 23231 micro bca reagent a ma 23232 micro bca reagent b mb 1859068 micro bca reagent c. Piercea protein assay kit bc thermo fisher scientific. Reagents bicinchoninic acid solution, catalog number b9643 reagent a is a 1,000 ml. Introduction the micro bca protein assay kit is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein.
The intensity of the blue complex is proportional to the amount of protein in the sample. The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. The micro bca protein assay kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature 60c, test tube procedure only. This modification of the bca protein assay is suitable for dilute protein samples 0. Biovisions bca protein assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents. The bca protein assay kit is a twocomponent, highprecision, detergentcompatible assay reagent set to measure total protein concentration compared to a protein standard. In literature they used micro bca assay to determine liposome associated proteins. The bicinchoninic acid bca protein assay is based on a twostep reaction in one tube.